Annealing Temp Calculator
PCR Annealing Temperature Calculator Forward Primer Sequence (5′ → 3′) Enter only A, T, G, C characters Reverse Primer Sequence (5′ → 3′) Calculation Method Basic (4°C×GC + 2°C×AT)Nearest Neighbor (More Accurate) Calculate Reset Recommended Annealing Temperature –°C Fwd Primer Tm – Rev Primer Tm – Fwd GC% – Rev GC% – The Annealing Temp…