Annealing Temperature Calculator
Annealing Temperature Calculator Primer Sequences Forward Primer (5′ → 3′) Reverse Primer (5′ → 3′) Primer Concentration (nM) Salt Concentration (mM) Formula Wallace Rule (Simple)GC Content MethodNearest Neighbor (Recommended) Calculate Reset Annealing Temperature –°C Suggested range: – to – Forward Primer Tm – Reverse Primer Tm – Fwd Length / GC% – Rev Length /…